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stlearn spatial transcriptomics analysis software  (Spatial Transcriptomics Inc)

 
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    Spatial Transcriptomics Inc stlearn spatial transcriptomics analysis software
    Developing and assessing protocols to perform spatial <t>transcriptomics</t> to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.
    Stlearn Spatial Transcriptomics Analysis Software, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stlearn spatial transcriptomics analysis software/product/Spatial Transcriptomics Inc
    Average 90 stars, based on 1 article reviews
    stlearn spatial transcriptomics analysis software - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Assessing spatial sequencing and imaging approaches to capture the molecular and pathological heterogeneity of archived cancer tissues"

    Article Title: Assessing spatial sequencing and imaging approaches to capture the molecular and pathological heterogeneity of archived cancer tissues

    Journal: The Journal of Pathology

    doi: 10.1002/path.6383

    Developing and assessing protocols to perform spatial transcriptomics to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.
    Figure Legend Snippet: Developing and assessing protocols to perform spatial transcriptomics to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.

    Techniques Used: Multiplexing, Staining, Reverse Transcription, Produced, Sequencing



    Similar Products

    stlearn spatial transcriptomics analysis software  (Spatial Transcriptomics Inc)
    90
    Spatial Transcriptomics Inc stlearn spatial transcriptomics analysis software
    Developing and assessing protocols to perform spatial <t>transcriptomics</t> to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.
    Stlearn Spatial Transcriptomics Analysis Software, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stlearn spatial transcriptomics analysis software/product/Spatial Transcriptomics Inc
    Average 90 stars, based on 1 article reviews
    stlearn spatial transcriptomics analysis software - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Developing and assessing protocols to perform spatial transcriptomics to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.

    Journal: The Journal of Pathology

    Article Title: Assessing spatial sequencing and imaging approaches to capture the molecular and pathological heterogeneity of archived cancer tissues

    doi: 10.1002/path.6383

    Figure Lengend Snippet: Developing and assessing protocols to perform spatial transcriptomics to capture thousands of genes in FFPE cancer tissue. (A) Poly(A)‐capture required the optimisation of tissue permeabilisation step. Probe‐capture required a tissue adherence test. (B) Optimisation for tissue multiplexing and sectioning thickness. (C) Tissue stainning was processed in different conditions in two protocols. (D) Decrosslinking was performed in the same way. (E) In permeabilisation, the mRNA molecules or hybridised probes were released from cells and bound to the spatial oligos on the glass slide. Reverse transcription produced cDNA products in poly(A)‐capture protocol or extended probes in probe‐capture protocol. (F) Eluting captured molecules/probes and preparing the library for long/short cDNA sequencing.

    Article Snippet: Datasets supporting this manuscript are available at Zenodo, DOI: https://doi.org/10.5281/zenodo.8053920 and code supporting this manuscript used our stLearn spatial transcriptomics analysis software available at https://stlearn.readthedocs.io/en/latest/ .

    Techniques: Multiplexing, Staining, Reverse Transcription, Produced, Sequencing